Suppression of proangiogenic factors of endothelial cells by Hemidesmusindicus.
Keywords:Hemidesmusindicus, angiogenisis, KDR, FLT 4, CD31 and VE Cadherin.
Hemidesmusindicus var. indicus (family: Asclepiadaceae) is a medicinal plant seen in
India. This plant is used as a medicine traditionally for treating ailments like biliousness,
blood diseases, diarrhea, respiratory disorders, skin diseases, syphilis, fever, bronchitis,
asthma, eye diseases, epileptic fits in children, kidney and urinary disorders, loss of appetite,
burning sensation and rheumatism. Than the use of H.indicusin traditional medicine as an
anticarcinogenic drug its mode of action on endothelial cells is not known. In the course of our
study with medicinal plants we found that extract Hemidesmusindicuspowder with M199 media
showed a significant antiangiogenic activity at a concentration of 500ng/ml. Since endothelial
cells showed significant decrease in proliferation in the presence of H. indicus, the expression
of angiogenic markers on endothelial cells in the presence of extract were quantified by real
time RT-PCR analysis. We selected CD 31, FLT-4, VWF, and VE cadherin as markers since all
of them are directly involved in angiogenesis and tumorogenesis. An overall down regulation of
angiogenic factors - KDR, FLT 4, CD31 and VE Cadherin was noticed in endothelial cell by the
treatment with the extract. From the obtained data we would like to suggest that the angiogenic
factors suppression may be the reason for its use in the treatment of carcinoma. The extract is a
combination of different soluble fractions, hence a broad study is required for identifying the
active component from this plant involved in angiogenesis.